Protein engineering, folding, prediction, and design make up Chapter 5. Evolution of new function is discussed in terms of divergence, recruitment, domain duplication, and swapping. Globins, NAD-binding domains of dehydrogenases, and visual pigments are detailed examples. Structure is discussed vis a vis catalysis, conformational changes, and regulation of function, with hemoglobin used as a detailed example.Ĭhapter 4 deals with evolution of protein structure and function, structure and domain classification. It includes the basics of protein chemical structure and bonding, spectroscopic methods, and introduces x-ray crystallography, nuclear magnetic resonance, and cryo electron microscopy. Chapter 2 finishes with introductions to proteomics, mass spectrometry, microarrays, and bioinformatics.Ĭhapter 3 covers chemical structure and activity of proteins in about 50 pages.
A clear and very useful distinction is drawn between “similarity” and “homology,” giving examples. Students will be engaged when some of the examples highlight the clinical consequences of mutations, relating them to structural consequences. Alternate splicing, post-translational changes, primary, secondary, tertiary, and quaternary protein structure, topology, domains, and chaperones are explained, followed by a survey of functional and structural categories of proteins, each with examples. The title, Genomics and Proteomics, may be somewhat of a misnomer, because only the first few of 55 pages deal with genomics, and the chapter gets to proteomics in the strict sense only at the end. The ribosome is a ribozyme.”Ĭhapter 2 strongly emphasizes protein three-dimensional structure. “Cells take ribosomes very seriously.” (Did you know that the 15,000 ribosomes in each Escherichia coli cell constitute 25% of its mass?) About the crystal structure of the ribosome: “The results came as a shock…. Thus, the present work, Introduction to Protein Science, complements rather than competes with more specialized treatments of protein structure, such as Lesk's Introduction to Protein Architecture (Oxford, 2001) or Introduction to Protein Structure by Carl Branden and John Tooze (Garland, 2nd Edition, 1999).Ĭhapter 1 offers a lovely brief introduction to the central dogma, with emphasis on ribosome structure and function, with attention to history-in only 10 pages. It provides a broader and less specialized starting point for two previous books by Lesk, Introduction to Protein Architecture and Introduction to Bioinformatics, thereby completing a trilogy.
This book provides a lucid, authoritative, multi-disciplinary, yet succinct introduction to the science of proteins.
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